High-Performance Size Exclusion Chromatography Using Enhanced-Fluidity Liquid Mobile Phases

Enhanced Fluidity Liquid Chromatography

High performance liquid chromatography (HPLC) is often the method of choice for the separation of nonvolatile solutes. LC commonly exhibits longer analysis times, larger pressure drops and lower efRciency than either supercritical Suid chromatography (SFC) or gas chromatography (GC). The greater viscosities and lower diffusivities of liquids compared to those of supercritical Suids or gases are...

Organic matter from biofilter nitrification by high performance size exclusion chromatography and fluorescence excitation-emission matrix

A combination of high performance size exclusion chromatography with organic carbon detector and ultraviolet detector coupled with peak-fitting technique and fluorescence excitation-emission matrix spectrometry applied fluorescence regional integration method was conducted to determine the characteristics of organic matter during nitrification. The batch scale of bionet nitrification without or...

Superheated Water Mobile Phases: Liquid Chromatography

Coupled SFE}SFC has shown itself to be a very useful technique for those samples for which it is applicable. The ability to transfer all the extract to the analytical column without manipulation increases sensitivity, reduces contamination and sample handling. The overloading of capillary columns is avoided. Now that methods for using modiRers in the extraction solvent and SFE sample injection ...

UNIFIED pH OF LIQUID CHROMATOGRAPHY MOBILE PHASES

A hemagglutinin quantification method for development of an influenza pandemic vaccine using size exclusion high performance liquid chromatography.

Single radial immunodiffusion (SRID) assay requires a reference antigen and an antibody to the hemagglutinin (HA) of an influenza vaccine. As it takes 2‑3 months to develop the reference antigen, vaccine development is delayed in cases of an influenza pandemic. In the present study, the measurement of the HA content of influenza vaccines was assessed using size exclusion high performance liquid...